In part 4, Prof. Caplan talks about isolating mesenchymal stem cells from bone marrow using specialized; calf serum choosing different assays to prove multipotency – osteogenesis, chondrogenesis, adipogenesis; point of care with autologous bone marrow in orthopedic surgery; tissue engineering bone with lineage restricted MSCs; banking bone discarded bone marrow from orthopedic surgeries for future use;
VIDEO – The Science of Mesenchymal Stem Cells and Regenerative Medicine – Arnold Caplan PhD (Part 4)
In part 3, Professor Caplan discusses the science behind mesenchymal stem cells: sources of mesenchymal stem cells (MSCs), because all MSCs are pericytes one can find them in any tissue that has blood vessels, pericytes express markers of MSCs, frequency of pericytes in human tissue, most abundant source of pericytes is adipose (fat) tissue, adipose-derived stem cells, how MSCs are separated from fat, chemistries MSCs from different tissues are not the same, MSCs function at sites of injury, mesenchymal stem cell homing in mice, MSCs don’t make fat, they don’t make muscle. They come back as pericytes, and not all pericytes are MSCs.
Professor Arnold Caplan of Case Western Reserve University is widely regarded as “The Father of the Mesenchymal Stem Cell”. This lecture is a “must see” for anyone interested in stem cell therapy. In Part 1, Prof. Caplan proposes a new regulatory pathway for approval of cell-based therapies and regenerative medicine called “Progressive Approval” to replace the current US FDA system that is now in place.
Prof. Caplan was speaking in Panama City, Panama at “La Medicina Del Futuro En El Presente”, an event organized by the honarable Ruben Berrocal MD, Minister of Science, Technology and Innovation SENACYT (National Secretariat of Science, Technology and Innovation) and Prof. K. S. Jagannatha Rao, Ph.D., FNASc, FABAP, FASB, FLS (Reino Unido) Director INDICASAT-AIP (Instituto de Investigaciones Cientificas y Servicios de Alta Tecnologia — Institute for Scientific Research and High Technology Services).
Arnold Caplan PhD of Case Western Reserve University and Riccardo Calafiore of Perugia University in Italy tour Medistem stem cell lab in Panama
Professor Arnold Caplan (left) and Professor Riccardo Calafiore (right) pose with Medistem Labs Panama Founder, Neil Riordan, PhD. Dr. Riordan is also the Founder of Stem Cell Institute in Panama City, Panama.
Prof. Caplan and Prof. Calafiore were in Panama City with Amit Patel MD to speak at “La Medicina Del Futuro En El Presente”, an event organized by the honarable Ruben Berrocal MD, Minister of Science, Technology and Innovation SENACYT (National Secretariat of Science, Technology and Innovation) and Prof. K. S. Jagannatha Rao, Ph.D., FNASc, FABAP, FASB, FLS (Reino Unido) Director INDICASAT-AIP (Instituto de Investigaciones Cientificas y Servicios de Alta Tecnologia – Institute for Scientific Research and High Technology Services).
Prof. Caplan is a Professor of Biology and General Medical Sciences (oncology) at Case Western Reserve University and the Director of the Skeletal Research Center at Case Western Reserve. Prof. Caplan is widely regarded as “The father of the mesenchymal stem cell”.
Prof. Calafiore is the Head of the Division of Endocrinology and Metabolism at the Medical School at the University of Perugia, Italy and Director of the Interdisciplinary Laboratory for Endocrine and Organ Transplant at the University of Perugia School of Medicine. He is also a director at ALTuCELL.
Amit Patel, MD, MS, is an associate professor in the Division of Cardiothoracic Surgery at the University of Utah School of Medicine and Director of Clinical Regenerative Medicine and Tissue Engineering at the University of Utah
King Goff received three applications of his own adipose tissue-derived stem cells over the course of 3 days for a knee injury and autoimmune issues at the Stem Cell Institute in Panama. In this video, Mr. Goff discusses conventional treatments he received before stem cells, pre-treatment symptoms, post-treatment improvements, the doctors and staff, and the clinic.
“My immune system is the thing that is noticeably better. My allergies have corrected. The sinusitis circumstances that I was having problems with have corrected, in part but not completely. My knees, I’m up to being able to be on fairly distant walks of one and a half to two miles as part of my exercise program without the pain that I experienced prior to the [stem cell] treatment. Those are the most significant gains that I can say; just a lack of pain and increased mobility.” – King Goff
A short film about three spinal cord injury patients who share their stories of tragedy, hope and recovery following stem cell treatments for spinal cord injury at the Stem Cell Institute in Panama City, Panama. See them demonstrate their improvements by lifting themselves up, standing and even walking after stem cell therapy.
Spinal cord injury patient, Chris Niles, discusses his improvements after undergoing stem cell therapy at the the Stem Cell Institute in Panama City, Panama. Chris now has sensation down to about a T10 level and has regained movement in his feet.
Theo Kostoulas is a T-6 complete spinal cord injury patient. He received a course of 16 stem cell therapy injections using umbilical cord-derived stem cells and his own bone marrow-derived stem cells. The cells were injected both intravenously and intrathecally (into the spinal fluid). During his stay in Panama and as part of his treatment protocol, Theo went to 19 physical therapy sessions over the course of month.
“I can definitely say that after the second injection, I immediately actually started getting some leg sensation and touch back from below my injury level. It was me lying in bed while my mother was putting my sock on my feet. I was actually able to feel that and it was pretty amazing because I haven’t had and feeling or sensation at all from my injury level of T-6. Now, more towards the end here, I have actually been able to have bladder sensation. I am a person that usually cath’ed myself every four hours but now because I do have this sensation coming back, I tend to wait until I have the need to have to go. So it can be anywhere from six to seven hours. If I need to drink a lot of water before coming to treatment, I know ahead of time whether I need to cath myself sooner or not.”
Michelle Kéramidas, Florence de Fraipont, Anastassia Karageorgis, Anaïck Moisan, Virginie Persoons, Marie-Jeanne Richard, Jean-Luc Coll and Claire Rome
Understanding the multiple biological functions played by human mesenchymal stem cells (hMSCs) as well as their development as therapeutics in regenerative medicine or in cancer treatment are major fields of research. Indeed, it has been established that hMSCs play a central role in the pathogenesis and progression of tumours, but their impact on tumour growth remains controversial.
Our results suggest that hMSCs injection decreased solid tumour growth in mice and modified tumour vasculature, which confirms hMSCs could be interesting to use for the treatment of pre-established tumours.
In this study, we investigated the influence of hMSCs on the growth of pre-established tumours. We engrafted nude mice with luciferase-positive mouse adenocarcinoma cells (TSA-Luc+) to obtain subcutaneous or lung tumours. When tumour presence was confirmed by non-invasive bioluminescence imaging, hMSCs were injected into the periphery of the SC tumours or delivered by systemic intravenous injection in mice bearing either SC tumours or lung metastasis.
Regardless of the tumour model and mode of hMSC injection, hMSC administration was always associated with decreased tumour growth due to an inhibition of tumour cell proliferation, likely resulting from deep modifications of the tumour angiogenesis. Indeed, we established that although hMSCs can induce the formation of new blood vessels in a non-tumoural cellulose sponge model in mice, they do not modify the overall amount of haemoglobin delivered into the SC tumours or lung metastasis. We observed that these tumour vessels were reduced in number but were longer.
Our results suggest that hMSCs injection decreased solid tumour growth in mice and modified tumour vasculature, which confirms hMSCs could be interesting to use for the treatment of pre-established tumours.
Original Link: http://stemcellres.com/content/4/2/41/abstract
April 17, 2013
I watched my father deteriorate through the years progressing from walking with a cane, spring assist in his shoes to lift the toe, to hand crutches, his inability to control his bladder and bowels, to being permanently bound to a wheel chair by 1979. Building all sorts of ramps, elevators and installing van lifts so that he could be able to get around by himself. He is still alive and wearing out electric wheel chairs to this day.
I had a few problems with my eyes in 1981, which they tested me for MS knowing my father’s history, but everything at that time came up negative (no MRIs used at that time). I then had a problems with my eyes & speech (blurry & slurring – which dissipated that day) in the summer of 2003, which the doctors preliminary diagnosis was a mild stroke, but then the test began. The outcome came on November 10th, 2003 from my neurologists – you have MS. This just about devastated me having watched the progression (walking to not walking, loss of bowel & bladder control, and loss of mobility & arms, etc.).
Well, they started me on Betaseron, and then the fun began. The first nine (9) months on Betaseron was a roller-coaster of emotions, periods of not having any concentration, and not being able to remember how to do little things that I’ve done for twenty some-odd years. Thank “GOD” for my loving wife (Kim) for getting me through this time..
After making it through the first nine months, things settled, but the MS progressed. Legs burning, legs jerking, walking becoming difficult, hands trembling, lots of floaters in my eyes and twitching. Continued my appointments with my neurologists and as things progressed, more meds prescribed.
In April of 2009, they conducted more test including an Evoke Potential (hooked me up to electrodes and actually got me to levitate off of a table when the zapped me) and more MRIs, etc. These test resulted in a diagnosis of Myasthenia Gravis in conjunction with the MS. More meds prescribed.
In the fall of 2011, walking was very difficult, writing was a joke (if someone other than myself was reading it), having lots of bladder and bowel issues (everything seemed to be leaking) and if I had to travel with my job, I had be chauffeured, as I couldn’t make the trips by myself. Hot weather made me wilt like a cut flower out of water. With all of this going on with my body, the neurologists ordered up another batch of test and then concluded that Betaseron had out lived its effectiveness and I was changed to Copaxon.
After a month of Copaxon, I was having whelps and redness appear at the injections site, which I called the folks at Copaxon and my neurologists and explained what was happening. Both told me it just took a little while for my body to adjust and they would go away. Another month passed and I was doing the injections in my thigh, when the swelling and inflammation became so bad I couldn’t hardly put on my jeans and could hardly walk, so I marched down to my neurologists and sat in her office until she could see me. She took one look at the sites and said that I was allergic to Copaxon, and changed the meds to Avonex – oh joy. I had to undergo 21 days of steroid treatment prior to beginning Avonex.
It’s now February of 2012 and things are not getting any noticeably better, or slowing down, and I’m getting fed up and not looking forward to what I have seen with my father.. I keep taking the meds and start investigating my options.
A friend had mention to my wife about stem cell therapy that they had heard about down in Costa Rica. We researched it and found it in Panama, started checking it out and we liked what we read..
I submitted the initial applications in late March and received approval in April (just 7 days later). Just so happened that The Stem Cell Institute – Panama was having a seminar that month in Gilbert, AZ, so we signed up and flew to Phoenix (we wanted a little more comfort factor). We were very impressed & actually was able to talk with the doctors (Neil Riordan and Dr. Paz Rodriguez) and go over everything (also met one person that had been through the treatment that had a spinal cord injury, one that has MS and both where doing pretty good, as their stories were documented on film and a part of the presentation) .
The seminar was very good and we were sold on the fact that we had made the right decision. .
After arriving back home, we submitted for a treatment to get in the queue’ (which we were told would take as long as 5 months to find an opening), and received notification that it would be on May 14th, 2012, so we confirmed the reservations and began preparing for our trip.
At this time I had quit smoking and the current list of drugs was as follows:
- Amantadine: 100mg (2 tablets in AM)
- Effexor: 75mg (1 tablet daily)
- Zanaflex: 4mg (1/2 in AM & 1 at night)
- Plavix: 75mg (once daily)
- Crestor: 5mg (1 tablet at night)
- Mestinon: 60mg (1 tablet in 4 times daily)
- Mirapex: 0.125 mg (30 minutes before bedtime)
- Ampyra :10mg (1 tablet in am & 1 tablet in PM
- Avonex: 30mcg injection (once a week)
A week prior to our trip to Panama I had a regularly schedule appointment with my neurologists and at the end of the office visit I informed her of my intentions of going to Panama. I did not receive any negative response, she just want to make sure of the procedure that was going to be conducted and where the stem cells were coming from. She also relayed that she was an investigator for stem cell research for the State of Texas.
Here’s a log of the adventure to Panama. Anxiety is high
Flew to Panama from Amarillo. Left Amarillo at 9:30AM & arrived in Panama at 7:30PM (had a little trouble with my legs from Houston to Panama – spasms). We were met by Stem Cell Institute (SCI) representative at the airport & walked though customs, like you have never seen. Escorted through and express line at customs, escorted us to a lounge and then they went to go retrieve our luggage. SCI had transportation set up from the airport to your hotel, via private courier.
Picked up at the hotel by SCI and taken to the SCI office & met with 3 doctors and had blood taken (11 vials). All the doctors were great (Dr. Rodriguez, Dr, Hernandez and Dr. Bowen) and explained everything in great detail. Discussed the surgery (mini-liposuction the next morning). The stem cells were harvested from my own body – no umbilical cord cells utilized. SCI driver took us back to the hotel.
Picked up by SCI and taken to the hospital @ 8:00AM. A little anxious about the facilities that we were going to, but when we drove up the signage on the building read “Hospital Punta Pacifica, affiliated with John Hopkins Medicine International”.- no more worries. I was met at the door by Dr. Bowen (from Stem Cell), admitted to hospital and surgery @ 10:00am, out of surgery recovery by 12:15pm, given pain pills & antibiotics, Dr. Bowen was in surgery & with me throughout the procedure. The plastic surgeon that performed the procedure was from the States. SCI driver takes us back to the hotel. Took only one pain pill for the entire time. Have to wear a girdle (Velcro & elastic) for twenty-one days.
SCI picks us up at 9:30AM for 10:00am appointment for physical therapy. Stretching exercises and a small work out. Driver takes us back to hotel.
Same as yesterday
Same as yesterday.
Took a tour to the rainforest & the lake on the top of the canal. Saw, sloths, monkeys, butterflies coatis, toucan & crocodiles (all in the wild) – (gone 6 hours)
Took a tour to the waterfall and zoo. (gone 7 hours)
5/21 (Monday): SCI picks us up at 9:30AM for 10:00am appointment for physical therapy. Last day of physical therapy. Diver takes us back to hotel. Kim found a casino at the Sheraton Inn & we went for a little while.
Took a tour through the Panama Canal Locks & up to the lake. We met a couple of others folks at the motel that evening that were down from the States to have the treatment. One gentleman had come down for his second set of treatments and was expounding as to how great his progress had been, and was hoping that with this set that he could be able to walk without a cane. His first set of treatments was about eight months prior. Ok, now I’m getting very anxious.
SCI picks us up at 9:30AM for 10:00am appointment to receive 1st infusion of stem cells. Received 20cc which took about 15 minutes, once I got back to the examination room. . Driver took me back to the hotel – back before 10:30AM. After lunch, Kim asked if I was feeling any effect from the first treatment and I barked back that it takes months for the effects to take place (sorry that I made that comment). Kim had inquired and found another casino, so we took a trip to the Wyndum Casino. After about 2 hours (at about 8:00PM) I noticed that I wasn’t limping, or staggering, dragging my foot, or any of that other good stuff – I went to find Kim. Found Kim & asked her what she thought about my walking and she was really surprised.
SCI picks us up at 10:15AM for 10:30am appointment to receive 2nd infusion of stem cells. Told the Dr. (Randy Bowen) about my walking and he was very surprised. I also relayed that the floaties that I have had in my eyes are diminishing, the tingling in my hands is diminishing & my feet don’t feel like they are on fire, bladder control is doing better. Driver took me back to the hotel. Had lunch and then started just messing around and found that I could balance on one leg (either leg) with my hands in my pockets (been quite a while since I was able to balance like that, even without my hands in my pockets) Went to the casino for a little while.
SCI picks us up at 9:30AM for 10:00am appointment to receive 3rd infusion of stem cells from the Dr. (Hernandez) & he said that he had heard from Dr. Bowen about my results, so I showed him the balance act which he was very impressed with. Both He & Dr. Bowen stated that I was fortunate to have results so quickly, but should continue receiving more results up to at least 6 months. They asked that I keep them apprised of any and all progress. Received pack of documentation covering everything that was done by SCI (complete blood analysis & certification of 6.6*E7 >75% viable stem cells). They also apprised me that they have retained 50% of the stem cells harvested for utilization at a future date – which is only a one week trip. Dr. Hernandez said that I could start decreasing my medications gradually. (Driver took me back to the hotel. Off we go to the Wynndum Casino for a couple of hours. Walking is getting better.
Tired today, slept until 11:45AM, then went to grab lunch, laid back down at 1:30PM and slept until 4:30PM. Went and grabbed a few beers at the hotels bar. I haven’t been able to walk, or see like this in 6 years.
SCI Driver pick us up at 7:00AM and assisted through the line at the airport. Arrived back in Amarillo at 8:00PM. Had a little trouble with my legs (Spasms) from Houston to Amarillo, but nothing like it had been for the past 5 years.
Went to the shop & office – didn’t do much. Had supper with our daughters and they couldn’t believe how I was walking. My wife insisted that I show them the balancing act. Girdle & levis, don’t go together very well.
The facilities and doctors are really great. Very professional, very clean, very informative and they help you with anything that you need.
With the results that I was seeing in the first week, I stopped taking a lot of my meds.
On June 16th we took a trip and drove to Ft. Smith, AR (444 miles) to attend a barbeque cook for my friends. Amazingly I had no spasms or leg jerks on the trip over or back, and I tolerated the heat exceedingly well. The only meds that I was still taking at this juncture was Zanaflex and Effexor.
In mid-July I was sleeping like a rock and awakening maybe just once an evening. No more one hour sleeps and then run to the bathroom – no more leg jerks – no more spasms. I had also traveled to one my friends ranch and was out in +97° weather and had no problems.
The first part of August, Kim had purchased a guided fly fishing float trip in Oregon. Ok, now I’m a little skeptical, but here I go. We fished for five days out of boats, floating down the rivers with fly rods. You had to stand up in the boats that were moving with the currents and going through rough water. Amazingly, I never lost my balance. Life is good. Completely off of ALL meds.
After arriving back from Oregon, I had a scheduled appointment with my neurologist. I informed her that I was totally off all of my meds, which she raised an eyebrow upon, then she ran me through the paces for balance, strength, eyes, and walking. She seemed impressed but didn’t say a whole lot, but what she said I really loved. I was discharged from all follow-ups and associated test until such time as I felt that I needed to see her.
The Stem Cell Institute still calls periodically (and sends questionnaires) to check on my conditions and inquire if I need anything additional, which is great. I have very few doctors, or clinics, that have ever called to check on me.
At the writing of this, I am one month from my one year anniversary of the treatment and I cannot express what a change in life it has been. I’m no longer in pain. I’m not irritable. I’m happy. I can walk. I don’t have to worry where the restrooms are located. I can jog (if I wanted too). I can work outside in the summer. My family and friends are astonished with my progress. It is like you rolled the life clock back twenty years.
If this procedure was available fifteen years ago, my father would not have had a say. I would have loaded him on a plane and he would have gone to Panama,
I strongly recommend this procedure and The Stem Cell Institute. They and their staff treat you like a king and you become part of their family. It is life changing Thank “GOD” for the pioneers in medicine.
Anyone reading this that would like to discuss my progress, or any of the above, may feel free to contact me at (Number available upon request to all approved applicants).
Kelley R. Wilson.