Stem Cell Related Patent Number US7081336

Dual resonance energy transfer nucleic acid probes

Bao, Gang; Mableton, GA, United States of America
Tsourkas, Andrew; Atlanta, GA, United States of America
Xu, Yangqing; Atlanta, GA, United States of America

This invention describes a composition for detecting target nucleic acid via dual nucleic acid probes with resonance energy transfer moieties. Described herein are fluorescent or luminescent resonance energy transfer moieties on hairpin stem-loop molecular beacon probes that hybridize sufficiently near each other on a subject nucleic acid, such as mRNA, thereby generating an observable interaction. Further disclosed are lanthanide chelate luminescent resonance energy transfer moieties on linear and stem-loop probes that hybridize in a similar manner, thereby providing detectable signals for the rapid, specific and sensitive determination of hybridization in vivo. Applications are provided in the detection of nucleic acid target hybridization, as are therapeutic uses in assessing drug candidates, and in the genetic analysis of various diseases such as cancer.

Application Number: US2002000179730

Date Filed: 06/25/02

Dated Published: 07/25/06

Assignee: Georgia Tech Research Corporation, Atlanta, GA, United States of America