Stem Cell Related Patent Number US6664107

CD45 disrupted nucleic acid

Mak, Tak Wah; Toronto, Canada
Kishihara, Kenji; Fukuoka, Japan

This invention introduces a nonhuman mammal and its progeny with suppressed expression of the gene encoding CD45 on B cells. Specifically, the authors describe a mouse lacking expression of a particular CD45 isoform in certain cells of the immune system, such that the mouse may optionally contain a transgene encoding the CD45RO isoform. Further disclosed is a DNA sequence comprising at least exons 6-8 of a mouse CD45 DNA construct into which a marker gene sequence has been substituted for at least exon 6 and intron 6. Specific DNA sequences are delineated, as are applications in the screening of drugs for their immunomodulatory effects.

Application Number: US1994000191225

Date Filed: 02/02/94

Dated Published: 12/16/03

Assignee: Ontario Cancer Institute, University Health Network, Toronto, Canada