Stem Cell Related Patent Number US6218186

HIV-MSCV hybrid viral vector for gene transfer

Choi, John Kim; Boothwyn, PA, USA
Gewirtz, Alan M.; Narberth, PA, USA

This invention describes a hybrid viral vector for the transfer of selected genes to cells and mammals, such that the vector is a hybrid of a human immunodeficiency-based lentivirus and a murine stem cell retrovirus. Introduced herein is a hybrid viral vector comprising a human immunodeficiency virus and a murine stem cell retrovirus wherein the 3′ long terminal repeat of the lentivirus is replaced by a modified 3′ long terminal repeat comprising 50 base pairs of a 5′, end of the human immunodeficiency virus U3 region, a complete murine stem cell retrovirus long terminal repeat, and 50 base pairs of a 3′ end of the human immunodeficiency virus U3 region followed by a complete human immunodeficiency virus RU5 region, which are described as being operably linked in that order.

Application Number: US1999000439000

Date Filed: 11/12/99

Dated Published: 04/17/01

Assignee: Trustees of the University of Pennsylvania, Philadelphia, PA, USA