Stem Cell Related Patent Number US6150584

Human antibodies derived from immunized xenomice

Kucherlapati, Raju; Darien, CT, USA

This invention describes a novel transgenic mouse which has been modified to produce fully human IgG2 heavy chains specific for desired antigen, IL-8, when immunized with IL-8, and whose endogenous loci has been disabled. Human antibodies against a specific antigen can be prepared by administering the antigen to a transgenic animal which has been modified to produce such antibodies in response to an antigenic challenge, but whose endogenous loci have been disabled. Various subsequent manipulations can be performed to obtain either antibodies per se or analogs thereof. This invention relates to a transgenic mouse wherein all of the somatic and germ cells comprise a DNA fragment of human chromosome 14 from the five most proximal VH gene segments, continuing through the D segment genes, the J segment genes and the constant region genes through C?d of the human immunoglobulin heavy chain locus, wherein this fragment does not contain a gene in question, and wherein the fragment is operably linked to a the corresponding human region gene.

Application Number: US1996000724752

Date Filed: 10/02/96

Dated Published: 11/21/00

Assignee: Abgenix, Inc., Fremont, CA, USA