Stem Cell Related Patent Number US6114598

Generation of xenogeneic antibodies

Kucherlapati, Raju; Darien, CT, USA
Jakobovits, Aya; Menlo Park, CA, USA
Kalpholz, Sue; Stanford, CA, USA
Brenner, Daniel G.; Redwood City, CA, USA
Capon, Daniel J.; Hillsborough, CA, USA

Described herein is a genome of embryonic stem cells which has been modified by yeast spheroplasts containing a yeast artificial chromosome comprised of a xenogeneic DNA segment. The invention relates to non-human mammalian hosts characterized by inactivated endogenous Ig loci and functional human Ig loci for response to an immunogen which produces human antibodies or analogs thereof. Products of the invention include chimeric and transgenic mice which exhibit stably integrated large, xenogeneic DNA segments which have been introduced by fusion with yeast spheroplasts comprised of yeast artificial chromosomes (YACs) and which include the xenogeneic DNA segments as well as a selective marker such as HPRT, and embryonic stem cells. Therapeutic applications are included for the testing of toxicity and drug development, in the development of derivative cell lines and in the isolation of genes or proteins involved in cellular differentiation. This invention represents a continuation of other applications filed by the same assignee and inventors.

Application Number: US1995000464582

Date Filed: 06/05/95

Dated Published: 09/05/00

Assignee: Abgenix, Inc., Fremont, CA, USA