Stem Cell Related Patent Number US6033907

Enhanced virus-mediated DNA transfer

Williams, David A.; Indianapolis, IN, USA

Described herein are methods of enhanced virus-mediated DNA transfer via the incorporation of fibronectin or fragments thereof. The invention relates to a process for increasing the efficiency of transduction of hematopoietic and other cells, thereby yielding a transduced population of viable mammalian cells, with the use of retroviruses according to a method of infecting the cells in the presence of fibronectin or fibronectin fragments that exhibit virus-binding and cell-binding domains. Claims of the invention include enhanced retroviral-mediated gene transfer by the fibronectin and fibronectin fragments into the cells, particularly the hematopoietic cells including committed progenitors and primitive hematopoietic stem cells, and improved methods for somatic gene therapy which capitalize on enhanced gene transfer. Therapeutic applications include increased transduction efficiency in gene therapy.

Application Number: US1995000536891

Date Filed: 09/29/95

Dated Published: 03/07/00

Assignee: Indiana University Foundation, Bloomington, IN, USA