Stem Cell Related Patent Number US6018096

Animal model for engraftment, proliferation and differentiation of human hematopoietic stem cells

Keating, Armand; Toronto, Canada
Wu, Dong-dong; Toronto, Canada

This invention introduces an animal model for the production of human tissue. Disclosed herein is a chimeric nonhuman genetically-immunocompetent mammal with a hematopoietic system consisting of cells of human origin and some lymphoid hematopoietic cells syngeneic to the mammal itself. The chimeric nonhuman genetically-immunocompetent mammal is selected from the group consisting of a mouse, a rat, a rabbit, a cat, a dog, a pig, a sheep and a non-human primate, wherein the hematopoietic system of the mammal consists of human passaged bone marrow stromal cells and human hematopoietic stem cells obtained from a CD34+ -enriched bone marrow fraction, such that the mammal contains transplanted syngeneic non-lymphoid spleen colony cells. Claims of the invention include human hematopoietic cells which may be obtained from the chimeric, non-human mammal. Therapeutic applications are included for the engraftment, proliferation and differentiation of human hematopoietic cells derived according to this model.

Application Number: US1993000055105

Date Filed: 05/03/93

Dated Published: 01/25/00

Assignee: Surrogen, Inc., Vancouver