Stem Cell Related Patent Number US6361997

Genetically modified CD34-negative adherently growing stem cells and their use in gene therapy

Huss, Ralf; 83666 Waakirchen, Germany

This invention introduces genetically modified, adherently growing CD34-negative stem cells. Described herein are very early, genetically modified hematopoietic and mesenchymal stem cells which are negative for the expression of the surface molecule CD34. Methods are provided by which the autologous CD34-negative adherently growing stem cell cultures from the peripheral blood of a patient are applied and efficiently tranfected or infected with genetic constructs, such that the gene products of these genes have been shown to provide long-term substitution for defective or absent proteins or factors in a patient. Therapeutic applications of such autologous stem cells include organ replacement and the transfer of gene therapy constructs for the treatment of immunodeficiency and metabolic disorders.

Application Number: US2001000743904

Date Filed: 02/24/01

Dated Published: 03/26/02

Assignee: None