Stem Cell Related Patent Number US6261549

Human mesenchymal stem cells from peripheral blood

Fernandez, Mireya; Santiago, Chile
Minguell, Jose J.; Santiago, Chile

This invention introduces methods for obtaining human mesenchymal stem cells from peripheral blood via the administration to a patient of at least one growth factor which increases the amount of mesenchymal cells that are recoverable from peripheral blood. Described herein are growth factors which preferably include G-CSF, GM-CSF and combinations thereof, along with the methods of use of such factors in the recovery of an isolated, culture-expanded population of human mesenchymal stem cells from the mesenchymal stem cell-enriched peripheral blood of an individual. Further disclosed are methods of preserving ex vivo such an isolated, culture-expanded population of human mesenchymal stem cells from peripheral blood, as well as methods for treating an individual with an isolated, culture-expanded population of human mesenchymal stem cells in the treatment of various diseases.

Application Number: US1998000108838

Date Filed: 07/01/98

Dated Published: 07/17/01

Assignee: Osiris Therapeutics, Inc., Baltimore, MD, USA