Stem Cell Related Patent Number US6194635
Embryonic germ cells, method for making same, and using the cells to produce a chimeric porcine
Anderson, Gary B.; Davis, CA, USA
Shim, Hosup; Davis, CA, USA
Described herein are methods of preparing ungulate embryonic stem-like cells via the culturing of primordial germ cells collected from an ungulate embryo. The invention relates to methods by which primordial germ cells are extracted from post blastocyst porcine embryos by extracting primordial germ cells from the gonadal ridges of 25-day-old porcine embryos. After culturing, the primordial germ cells yield to cells which resemble embryonic stem cells in morphology and pluripotency. In one advantageous embodiment of the invention the cells are maintainable for several months in culture and can be genetically manipulated using homologous recombination technology in order to insert desired genetic material into the genetic complement of the cell at a specific location. Also provided is a genetically manipulated cell which can be inserted into a porcine blastocyst to produce a chimeric porcine. This invention represents a continuation of other applications filed by the same assignee and inventors.
Application Number: US1996000717155
Date Filed: 09/20/96
Dated Published: 02/27/01
Assignee: The Regents of the University of California, Oakland, CA, USA