Stem Cell Related Patent Number US6139835

Homologous recombination for allogeneic donor cells

Kucherlapati, Raju; Darien, CT
Koller, Beverly H.; Carrboro, NC
Smithies, Oliver; Chapel Hill, NC
Dubridge, Robert B.; Belmont, CA Greenburg, Gary; San Carlos, CA
Capon, Daniel J.; Hillsborough, CA
Williams, Steven R.; San Francisco, CA
De Rafael, Mariona Lourdes Arbones; Barcelona, Spain

This invention describes the use of homologous recombination to inactivate genes associated with antigenic expression, thereby yielding a cell line that may be used as allogeneic donor cells. The genetic engineering ex vivo of the mammalian cell line yields a diminished rejection response due to the lack of at least one functional Major Histocompatibility Complex (MHC) antigen. Methods for reducing and eliminating the expression of these Class 1 MHC antigens, as well as the precise methods by which homologous recombination is applied to the non-transformed mammalian somatic cells, are also described.

Application Number: US1995000443647

Date Filed: 05/18/95

Dated Published: 10/31/00

Assignee: Cell Genesys, Inc. (Foster City, CA, USA)