Stem Cell Related Patent Number US7119248
Antibodies against epitopes with homology to self antigens, methods of preparation and applications thereof
Rajewsky, Klaus; Cologne, Germany
Mueller, Werner; Cologne, Germany
Roes, Juergen; Cologne, Germany
This invention describes methods of production of autologous monoclonal antibodies (AMABs), or antibody fragments, that bind an epitope of a mouse self-antigen. Described herein is the use a genetically engineered host animal that does not biosynthesize at least one epitope of the antigen and utilizes the lack of self-tolerance of the host to the epitope to produce antibodies specific to the antigen. Further disclosed are methods of isolating cells comprising the use of such AMABs that have specificity for a cell surface antigen. Also provided are methods of immortalizing an antibody-producing cell to produce a hybridoma capable of continuously secreting an antibody that binds the self-antigen epitope.
Application Number: US1994000226168
Date Filed: 04/12/94
Dated Published: 10/10/06
Assignee: Miltenyi Biotec GmbH, Bergisch Gladach, Germany