Stem Cell Related Patent Number US7112576

Compositions and methods for cryopreservation of peripheral blood lymphocytes

Hubel, Allison; St. Paul, MN, United States of America

This invention comprises a novel composition and method for cryopreserving cells in a non-toxic medium, which is composed of the following: a balanced electrolyte solution, a cryoprotective agent that is arabinogalactan, or a biological or functional equivalent thereof, in an amount of 1% w/v to 40% w/v, a cryoprotective agent that penetrates the cell membrane, and freshly isolated lymphocytes, hematopoietic stem cells, lymphocytes which are modified ex vivo, or a combination thereof. The medium does not comprise dimethylsulfoxide or serum; the arabinogalactan, a biological or functional equivalent thereof, in the medium results in a high post-thaw survival rate for the freshly isolated lymphocytes, hematopoietic stem cells, or lymphocytes which are modified ex vivo.

Application Number: US1999000458862

Date Filed: 12/10/99

Dated Published: 09/26/06

Assignee: Regents of the University of Minnesota, Minneapolis, MN, United States of America