Stem Cell Related Patent Number US7118746

Conditioned cell culture medium compositions and methods of use

Naughton, Gail K.; Del Mar, CA, United States of America
Horwitz, David L.; San Diego, CA, United States of America
Applegate, Mark A.; San Diego, CA, United States of America
Zeltinger, Joan; San Diego, CA, United States of America
Mansbridge, Jonathan N.; La Jolla, CA, United States of America
Kern, Andreas; San Diego, CA, United States of America
Landeen, Lee K.; San Diego, CA, United States of America
Ratcliffe, Anthony; Del Mar, CA, United States of America
Pinney, R. Emmett; Poway, CA, United States of America

This invention describes novel components of a conditioned cell culture medium, and applications thereof. Methods of conditioning are described which employ a eukaryotic cell type such as stromal, parenchymal or mesenchymal cells, as well as liver reserve cells, neural stem cells, pancreatic stem cells or embryonic stem cells. A three-dimensional tissue construct is also disclosed, as are methods of genetic modification and properties of the conditioned medium, which may take the form of liquid, solid, frozen, lyophilized, dried and powdered embodiments. Also included are formulations with a pharmaceutically acceptable carrier as a vehicle for internal administration, as well as techniques for reducing deleterious effects to keratinocytes caused by intracellular oxidation. Therapeutic applications to wound healing are provided.

Application Number: US2000000979813

Date Filed: 05/12/00

Dated Published: 10/10/06

Assignee: SkinMedica, Inc., Carlsbad, CA, United States of America