Stem Cell Related Patent Number US7091035

Cell culturing and storage systems, devices and methods

Ravin, Rea; Rockville, MD, United States of America
Sullivan, James V.; Bowie, MD, United States of America
McKay, Ronald D.; Bethesda, MD, United States of America

This invention describes a novel long-term system of cell culturing, which is configured and arranged such as to allow the continual monitoring of the dynamic processes that occur during the proliferation and differentiation of stem cells. Further disclosed are aspects by which the cells may be manipulated by focal application of growth factors such as BMP and CNTF during the monitoring, at which time electrical recordings may be made from the cells. The particular microscopic viewing apparatus is disclosed, as are recording methods. Uses are explained in the long-term culturing of cells, such as central nervous system or embryonic stem cells

Application Number: US2002000334565

Date Filed: 12/30/02

Dated Published: 08/15/06

Assignee: The United States of America as represented by the Department of Health and Human Services, Washington, DC, United States of America