Stem Cell Related Patent Number US6001803

Composition of c-kit ligand, GM-CSF, and TNF-?a and method of use

Besmer, Peter; New York, NY, USA
Buck, Jochen; New York, NY, USA
Moore, Malcolm A. S.; New York, NY, USA
Nocka, Karl; Harvard, MA, USA

This invention describes properties and methods of use for a novel composition containing C-kit ligand (KL) with a hematopoietic factor and its anti-sense nucleic acid. Specifically disclosed herein is a pharmaceutical compound comprised of KL, GM-CSF and TNF-?a, the amount of each in the compound being such that the compound is effective in expanding and differentiating progenitor cells into dendritic cells. Methods are described by which the composition is either purified by applicants or produced by applicants’ recombinant methods in combination with other hematopoietic factors. Further provided are properties of a mutated KL antagonist and of a pharmaceutically acceptable carrier. Therapeutic applications of this pharmaceutical composition are also included, such as in the treatment of patients with leukaemia, anaemia, AIDS, cancer, and further described are uses of the mutated KL antagonists in the treatment of allergies.

Application Number: US1994000325240

Date Filed: 10/20/94

Dated Published: 12/14/99

Assignee: Sloan-Kettering Institute for Cancer Research, New York, NY, USA