Stem Cell Related Patent Number US7005299

Expression of heterologous genes according to a targeted expression profile

Smith, Austin Gerard; Edinburgh, United Kingdom
Mountford, Peter Scott; Elsderwick, Australia
Lathe, Richard Frank; Edinburg, United Kingdom

This invention describes a gene trap vector comprising a DNA construct containing an expression unit of an internal ribosome binding site (IRES) coupled to a heterologous gene sequence. Specifically, this method provides a means of inserting a gene of interest into an endogenous gene in a mouse embryonic stem cell such that the gene of interest is expressed in the mouse embryonic stem cell. The expression unit is used in gene trap protocols to obtain expression of the heterologous gene in the host. Precise coding and sequencing are disclosed.

Application Number: US1999000348469

Date Filed: 07/07/99

Dated Published: 02/28/06

Assignee: The University of Edinburgh United Kingdom