Stem Cell Related Patent Number US6884622

Method for preparing a mammalian cell deficient in HPRT

Jokobovits, Aya; Menlo Park, CA, United States of America

This invention describes a method by which mammalian cells are deficient in HPRT by genomic deletion. The method involves modification of the genome of the cell containing the wild-type locus via introduction of a construct comprising two regions of sequences which are homologous to 5′ and 3′ flanking the sequences of the targeted regions to be deleted in the locus. Further described are uses of a replacement-type targeting construct to delete large fragments of genomic DNA by such gene targeting. After transfection of the targeting construct into the desired cell line, gene targeted-mediated deletions are identified by selection and further characterized. Uses are recommended for various cases involving large genomic deletion. Examples of suitable loci are given to include MHC Class I and II antigens and immunoglobulin genes, such as the variable and constant regions of kappa, lambda, or heavy chains.

Application Number: US2000000718717

Date Filed: 11/22/00

Dated Published: 04/26/05

Assignee: Abgenix, Inc, Fremont, CA, United States of America