Stem Cell Related Patent Number US6114168

Active retinoic acid-free culture medium for chicken embryonic stem cells

Samarut, Jacques; Lyons, France
Pain, Bertrand; Lyons, France

This invention introduces a novel avian embryonic stem cell culture medium containing cytokines and growth factors but no retinoic acid. Further disclosed are genetically modified cultured cells and nonhuman transgenic mammals derived thereof. The invention relates to a medium for culturing chicken embryonic stem (ES) cells which is comprised of cytokines, fibroblast growth factors, insulin-like growth factors, stem cell growth factors and an anti-retinoic acid antibody, and which is substantially free of active retinoic acid. Therapeutic applications are included for the treatment of human disease by the antibodies produced by such transgenic mammals and other products of the invention described herein.

Application Number: US1997000817671

Date Filed: 05/12/97

Dated Published: 09/05/00

Assignee: Institute National de la Recherche Agronomique, Paris, France
Centre National de la Recherche Scientifique (CNRS), Paris Cedex, France
Ecole Normale Superieure de Lyon, Lyons, France