Stem Cell Related Patent Number US6040180

In vitro generation of differentiated neurons from cultures of mammalian multipotential CNS stem cells

Johe, Karl K.; Potomac, MD, USA

This invention introduces methods of propagation and the long-term in vitro culturing of central nervous system stem cells, such that the cells retain their differentiation capacity and exhibit selective differentiation into neurons, astrocytes and oligodendrocytes. Described herein are region-specific, terminally differentiated, mature neurons derived from cultures of mammalian multipotential CNS stem cells and an in vitro procedure by which the differentiated neurons may be generated. Further disclosed are methods by which neurons, astrocytes and oligodendrocytes may be generated from such an adhesion culture of mammalian multipotential CNS stem cells in a serum-free medium supplemented with a mitogen, the withdrawal of which renders such differentiation. Methods of harvesting the stem cells by trypsinization are disclosed, as are conditions under which the stem cells should be optimally plated at a density of between 100,000 to 250,000 cells per square centimeter. Therapeutic applications are included in gene and cell therapy and in the screening of various drugs and growth factors.

Application Number: US1997000919580

Date Filed: 05/07/97

Dated Published: 03/21/00

Assignee: NeuralStem Biopharmaceuticals, Ltd., College Park, MD, USA